The use of photographic materials as a dry reagent for determining the peroxidase activity of biological preparations
Aronbaev, D.M.; Varfolomeev, S.D.; Krivoruchko, V.I.; Simonova, M.V.
Meditsinskaia Tekhnika 1: 39-40
1990
ISSN/ISBN: 0025-8075 PMID: 2187138 Document Number: 354416
A new method is reviewed for measuring the peroxidase activity of biopreparations using light quantum-sensitized photographic material as a detector. The method involves the combination of the reactions of peroxidase oxidation of hydroquinone, a constituent part of the developing solutions, by hydrogen peroxide and photochemical interaction of the remaining hydroquinone with silver halogenides of the photoemulsion layer. Visually, up to 2 ng/ml of horse radish peroxidase is detectable. On photometry of the spots obtained, the limit of peroxidase demonstration amounts to 0.6 ng/ml. The data obtained during measurements of the peroxidase activity of the saliva, blood serum, plant extracts and immunoenzymic conjugates are provided.