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A photographic microfluorimetric method for determining the DNA content in individual human chromosomes

Sukhikh, T.R.; Kalimagambetov, A.M.; Barskiĭ, I.I.; Khomenkova, S.A.; Vinogradov, A.E.

Tsitologiia 31(11): 1329-1338

1989

ISSN/ISBN: 0041-3771
PMID: 2483280
Document Number: 330750
A method for determination of DNA contents in individual human chromosomes has been elaborated based of the two step analysis: 1) identification of chromosomes by Q-banding; 2) photographic microfluorimetry of chromosomes after the Feulgen staining (a fluorescent variant using a Schiff-type reagent of Auramine-SO2). The DNA content in 24 human chromosomes was calculated in absolute values (fg). The data obtained are compared with the evidence published elsewhere and provided by different cytophotometric methods.

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