Spectrophotometric and liquid chromatographic determination of dopamine from pharmaceutical preparations using acetyl acetone as derivatizing reagent
Khuhawar, M.Y.; Rajper, A.D.; Rind, F.M.A.
Pakistan Journal of Pharmaceutical Sciences 19(4): 286-289
2006
ISSN/ISBN: 1011-601X PMID: 17105705 Document Number: 597357
Dopamine (DA) has been determined by spectrophotometry and by liquid chromatography as derivatives of acetylacetone (AA). The liquid chromatography of DA was carried out in the presence of hydroxyproline (HP), octopamine (OP) and tyramine (TY). The separation was obtained from Phenomenex C-18 column 5 pm (150 x 4.6 mm id) by isocratic elution with methanol: acetonitrile: water (45: 6: 49 v/v/v), UV detection was at 320 nm. Linear calibration curves were obtained for each with 2.5-40.0 microgml(-1) with detection limits in the range 1.8-80 ng injection(-1) (10 microl). The dopamine was determined in pharmaceutical preparations with coefficient of variation (CV) within 0.7-1.5%.