Determining the peroxidase level using tubular coal electrodes in flow-type systems

Ivnitskiĭ, D.M.; Aronbaev, D.M.; Iulaev, M.F.; Kashkin, A.P.; Varfolomeev, S.D.

Meditsinskaia Tekhnika 5: 43-47

1987


ISSN/ISBN: 0025-8075
PMID: 2961969
Document Number: 286639
An amperometric transducer with tubular coal electrodes has been worked out to determine the microquantity of peroxidase. The hydrodynamic parameters of the transducer were studied as were the relationship of the output signal to the concentration of the electroactive substance, the length of the electrode, the speed of the background electrolyte movement and the diffusion coefficient. The line section of the relationship between the strength of current and the peroxidase concentration is observed within the interval of the enzymic concentration 1-32 ng/ml with the exposure time of the enzymic reaction of 5-10 min.

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