Potentiometric electrodes for determining choline, butyrylcholine and cholinesterase inhibitors
Gindilis, A.L.; Kurochkin, I.N.
Prikladnaia Biokhimiia i Mikrobiologiia 34(3): 326-331
1998
ISSN/ISBN: 0555-1099 PMID: 9644713 Document Number: 485565
Potentiometric choline electrodes were developed on the basis of the mediator-free bioelectrocatalysis. The electrodes made of a composite carbon-polymer material contain choline oxidase and peroxidase coimmobilized on the surface of the electrode. The rate of the potential increase was shown to be proportional to the choline concentration within a broad range of variation. Coupling of choline-sensitive electrodes with butyrylcholinesterase makes possible both the direct detection of butyrylcholine and analysis of butyrylcholinesterase inhibitors.