Effect of nucleophiles on the conversion of choline esters under the action of cholinesterase
Igumnova, N.D.; Bogatkov, S.V.; Cherkasova, E.M.
Biokhimiia 40(1): 63-70
1975
ISSN/ISBN: 0320-9725 PMID: 237583 Document Number: 85695
It is shown that effect of alcohols ROH on the hydrolysis of butyryl-choline and acetylcholine by choline esterase (E. C. 3.1.1.8) is complex; it included the concurrent and the unconcurrent inhibitions, the interaction with the acylenzyme to form more reactive triple complex, and the acyl migration. By titrometric method it is found that proportion of the transacylation and hydrolysis rates depends on hydrophobity of R only being independent on its electronegativity.