Fertilizing capacity of frozen boar semen following surgical insemination
Polge, C.; Salamon, S.; Wilmut, I.
Veterinary Record 87(15): 424-429
1970
ISSN/ISBN: 0042-4900 PMID: 5485635 Document Number: 23558
Boar semen in a yolk-glucose-glycerol diluent was frozen by the pellet method, stored in liquid nitrogen for 1-8 weeks and used to inseminate 58 gilts. Eleven animals were inseminated normally through the cervix and 47 by a surgical technique directly into the uterus or oviducts. The time of ovulation was controlled in all animals. Fertilization was determined by the examination of eggs 24 hours after insemination.
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