Study of the immunogenic properties of inactivated vaccines prepared with the infectious bovine rhinotracheitis virus (IBR)
Khristov, S.; Karadzhov, I.
Veterinarno-Meditsinski Nauki 13(3): 8-13
1976
ISSN/ISBN: 0324-1068 PMID: 181897 Document Number: 96718
Tested were the chemical and physico-chemical means for the inactivation of the infectious bovine rhinotracheitis virus. Formalin and saponin were shown to decrease the immunogenic activity of the virus. The inactivation of the virus, preserving its immunogenic properties, was achieved with the use of two inactivating factors (1 and 2), and the concentrated vaccines produced containing an oil adjuvant at amounts of 10 cu. cm per dose in the vaccination and revaccination at a 28-day interval proved highly immunogenic. Neutralizing antibodies in the vaccinated rabbits and calves were demonstrated after the 14th day following treatment. The antibody titers on the 28th day following vaccination, and on the 14th day following revaccination reached their peak levels: log 2.5 to 4.5 and 6.5 to 7.5, respectively. The problem is discussed of the correlation between the circulation and the secretory neutralizing antibodies after immunization with an inactivated IBR vaccine.