Biochemical studies on cytoplasmic membranes of agrocin sensitive and resistant strains of Agrobacterium tumefaciens
Basu, M.; Banerjee, D.; Tewari, R.; Das, P.K.; Chatterjee, G.C.
Acta Microbiologica Polonica 31(1): 37-49
1982
ISSN/ISBN: 0137-1320 PMID: 6184960 Document Number: 78
The cytoplasmic membrane of Agrobacterium tumefaciens accounted for 12-13,3/0 of the dry wt. of the whole cells and was found to consist of 71% protein, 24% lipid (of which 70010 was phospholipid) and 5-6% carbohydrates. The isolated cell membrane of A. tumefaciens was resolved into 15 protein bands by polyacrylamide gel electrophoresis, four of which stained for carbohydrates. The cytoplasmic membranes of the virulent agrocin-sensitive and avirulent agrocin-resistant strains of A. tumefaciens showed only minor quantitative variation of the protein bands in gel electropherograms. The membrane proteins of these strains were found to be antigenically identical as studied by immunodiffusion and immunoelectrophoresis and they cross-reacted with one another. The total lipid content of the membrane of agrocin-resistant strains was higher as compared with that in the normal strain. No appreciable differences were detected in the phospholipids, neutral lipids and fatty acids components of the whole cell and cytoplasmic membrane or among the cytoplasmic membrane fragments of the three strains studied.
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