Relationship between adenylate cytokinin production and Ti plasmid of Agrobacterium tumefaciens
Sonoki, S.; Ohno, Y.; Kijima, N.; Hishiyama, T.; Saito, H.; Sugiyama, T.; Hashizume, T.
Nucleic Acids Symposium Series 12: 111-114
1983
ISSN/ISBN: 0261-3166 PMID: 6664842 Document Number: 201979
To know whether the tumor-inducing plasmid of Agrobacterium tumefaciens carries genetic information of the biosynthesis of cytokinins, the levels of 6-(3-methyl-2-butenyl-amino)purine (iPAde) and its 4-hydroxy derivative trans-zeatin (trans-Z) and its p-beta-D-ribofuranoside (trans-ZR) produced in media by wild-type virulent strain, plasmid-cured avirulent strain and the deletion mutant were compared. The highest levels of iPAde and trans-Z were found in the culture filtrate of late-log phase growth of plasmid-containing virulent strain, then the levels of iPAde and trans-Z were reduced rapidly at stationary phase. The plasmid-cured avirulent strain and deletion mutant had low levels of iPAde and trans-Z throughout the growth. Results obtained here showed Ti plasmid plays an important role in cytokinin biosynthesis.