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Production of transgenic sugarbeet plants (Beta vulgaris L.) using Agrobacterium rhizogenes

Kishchenko, E.M.; Komarnitskiĭ, I.K.; Kuchuk, N.V.

Tsitologiia i Genetika 39(1): 9-13

2005

ISSN/ISBN: 0564-3783
PMID: 16018172
Document Number: 593373
Normal phenotype sugarbeet plants transformed with Agrobacterium rhizogenes were produced using direct regeneration from explants without hairy root phase. Kanamycin resistant plants and Ri-roots carrying the genes of neomycin phosphotransferase II and b-glucuronidase have been obtained. Integration of transgenes into sugarbeet genome was confirmed with GUS-assay and PCR using primers for the introduced genes.

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