The genetic transformation of Lycopersicon peruvianum var. dentatum by using Agrobacterium tumefaciens carrying a plasmid with the human beta-interferon gene

Rudas, V.A.; Piven', N.M.; Rivkin, M.I.; Vershinin, A.V.; Infante, D.C.; Rudas, V.A; Piven&apo; , N.M; Rivkin, M.I; Vershinin, A.V; Infante, D.C

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The genetic transformation of Lycopersicon peruvianum var. dentatum by using Agrobacterium tumefaciens carrying a plasmid with the human beta-interferon gene

Rudas, V.A.; Piven', N.M.; Rivkin, M.I.; Vershinin, A.V.; Infante, D.C.

Tsitologiia i Genetika 31(2): 17-22

1997

ISSN/ISBN: 0564-3783

PMID: 9157639

Document Number: 475918

Wild tomato plants of Lycopersicon peruvianum var. dentatum were transformed with pG11 plasmid having human beta-interferon (hIFN-beta) and NPTII genes. Transformation was demonstrated by polymerase chain reaction (PCR) and ELISA assay. Expression of hIFN-beta gene was identified by Western blot analysis. Transgenic plants produced seeds and F1 generation inherited integrated traits.