T-DNA transfer from Agrobacterium tumefaciens to the ectomycorrhizal fungus Pisolithus microcarpus
Pardo, A.G.; Kemppainen, M.; Valdemoros, D.; Duplessis, S.; Martin, F.; Tagu, D.
Revista Argentina de Microbiologia 37(2): 69-72
2005
ISSN/ISBN: 0325-7541 PMID: 16178458 Document Number: 588309
The model ectomycorrhizal fungus Pisolithus microcarpus isolate 441 was transformed by using Agrobacterium tumefaciens LBA1100 and AGL-1. The selection marker was the Shble gene of Streptoallotecius hidustanus, conferring resistance to phleomycin, under the control of the gpd gene promoter and terminator of Schizophyllum commune. Transformation resulted in phleomycin resistant clones which were confirmed by PCR to contain the resistance cassette. A. tumefaciens-mediated gene transfer would allow the development of RNA interference technology in P. microcarpus.