A diagnostic assay based on variable intergenic region distinguishes between Leishmania donovani and Leishmania infantum
Chocholová, E.; Jirků, M.; Lukes, J.
Folia Parasitologica 55(1): 75-78
2008
ISSN/ISBN: 0015-5683 PMID: 18578169 Document Number: 617922
We have developed a PCR assay that in a single reaction distinguishes between Leishmania infantum and Leishmania donovani strains on the basis of different size of the amplicon. The targeted intergenic region between putative biopterin transporter and nucleotide binding protein on chromosome 35 is highly variable, species-specific and can be amplified from clinical samples.
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