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Construction and immunogenicity study of DNA vaccine expressing human H5N1 influenza virus hemagglutinin

Huan, L-Fang.; Yao, L-Hong.; Chen, A-Jun.; Cheng, C-Sheng.; Jia, R-Qing.; Tian, Q.; Bo, H.; Guo, J-Qiang.; Wang, M.; Shu, Y-Long.; Zhang, Z-Qing.

Bing du Xue Bao 23(5): 366-370

2007

ISSN/ISBN: 1000-8721
PMID: 17969853
Document Number: 613682
Based on the first isolated human H5N1 influenza virus strain A/Anhul/1/2005 in China, HA and HA1 genes were amplified and cloned into the eukaryotic expression vector pStar. The recombinant plasmids pStar-HA and pStar-HA1 were transfected into COS7 cells. Western blot and IFA showed that the two recombinant DNA plasmids were successfully expressed in eukaryotic cells. BALB/c mice were immunized with the plasmids DNA by intramuscular injection. Anti-HA specific antibody in peripheral blood of immunized mice was tested by ELISA. The results showed that the recombinant plasmids successfully induced the anti-HA humoral immune response, and there was no significant difference between HA and HA1 as immunogen. This work provides basis for future development of novel avian flu vaccine.

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