Changes in the amino acid sequence of hemagglutinin during sequential adaptation of human influenza virus A to replication in mice
Grinev, A.A.; Samokhvalov, E.I.; Petrov, N.A.; Vasilenko, S.K.; Iuferov, V.P.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1988(9): 43-45
1988
ISSN/ISBN: 0208-0613 PMID: 3211187 Document Number: 309768
The primary structure of hemagglutinin (HA) gene of Influenza virus A/USSR/90/77 (H1N1) variants after 3 and 11 passages has been determined. In the HA1 coding region of mice-adapted virus (11 passages) there are two amino acid substitutions: Thr 89----Ala and Asn 127----Asp. At the first stage of adaptation (3-rd passage) only a single mutation was detected: Asn 127----Asp. The adaptation is accompanied by the loss of specific carbohydrate attachment sites adjacent to the receptor-binding site located at HA1 subunit with a concomitant variation in antigenicity.