Evaluation on construction and expression in vitro of nucleic acid vaccine of nucleoprotein of influenza virus a
He, J.-F.; Fang, S.-S.; Cheng, X.-W.; Zhang, R.; Deng, P.; Zhao, S.
Wei Sheng Yan Jiu 34(4): 419-422
2005
ISSN/ISBN: 1000-8020 PMID: 16229263 Document Number: 588259
Constructing nucleic acid vaccine of influenza virus A to study the protective effects. NP gene was reverse transcripted from influenza virus A and linked with pSECTAG 2/Hygro A to constructed nucleic acid vaccine of influenza virus A. The constructed nucleic acid vaccine was transinfected into VERO cell resorting to lipofectamineTM2000. NP gene of influenza virus A was expressed in VERO cell by the method of ELISA. The results showed that the NP gene was expressed to the highest level at 36h after transinfection with the A40 value of 0.382. From 36 h to 60 h after transinfection, the expression of NP gene was stable (the A45, value at 48h and 60h was 0.385 and 0.387, respectively) . The nucleic acid vaccine of influenza virus A was successfully constructed, which sets up groudworks for the research of effection of nucleic acid vaccine in vivo.