Top Abstract Purchase Related
Home
>
Node
>
Subnode

Proteolytic cleavage of influenza virus matrix protein and nucleoprotein in an in vitro system

Iaroslavtseva, N.G.; Sokolova, M.V.; Khristova, M.L.; Grigor'ev, V.B.; Kharitonenkov, I.G.

Voprosy Virusologii 28(5): 549-556

1983

ISSN/ISBN: 0507-4088
PMID: 6362200
Document Number: 207677
Proteolytic modification of NP-protein which is a part of influenza virus ribonucleoprotein (RNP) was studied in vitro. NP-protein of influenza virus (mol. weight 56 000) was shown upon RNP treatment with bromeline to undergo proteolytic cleavage revealing 2 more polypeptides with molecular weights of 53 000 and 45 000 daltons. The same classes of NP-proteins were present in RNPs recovered from subviral units obtained by virus treatment with bromeline at buffer pH below 7. Electrophoresis in 12% polyacrylamide gel under reducing conditions of subvirus units obtained by treatment of virions with bromeline at the buffer pH below 7 also demonstrated proteolytic cleavage of M-protein present in them into fragments with molecular weights of 8 500-12 000 daltons. Similar results were obtained upon direct treatment with bromeline of M-protein isolated from virions.

Document emailed within 1 workday
Secure & encrypted payments

Khristova, M.L.; Busse, T.L.; Egorenkova, E.M.; Leonov, S.V.; Sokolova, M.V.; Gitelman, A.K.; Herrmann, J.; Döhner, L.; Kharitonenkov, I.G. 1989: Antigenic reactivity of matrix protein and nucleoprotein of influenza virus as detected by EIA after dissociation with different detergents Acta Virologica 33(1): 1-7
He, J.-F.; Fang, S.-S.; Cheng, X.-W.; Zhang, R.; Deng, P.; Zhao, S. 2005: Evaluation on construction and expression in vitro of nucleic acid vaccine of nucleoprotein of influenza virus a Wei Sheng Yan Jiu 34(4): 419-422
Sovová, V.; Trávnícek, M.; Hlozánek, I.; Cerná, H.; Sulová, A. 1984: Analysis of gag-myc proteins of partially transformation-defective mutants of avian myelocytomatosis virus strain MC29 by in vitro cleavage with protease p15: indication for the presence of specific cleavage site p15 in the myc domain of fusion protein Folia Biologica 30(3): 145-151
Dobretsov, G.E.; Zakomyrdin, I.A.; Spirin, M.M.; Booker, D.; Kharitonenkov, I.G. 1982: Localization of the tryptophan residues of influenza virus matrix protein in reconstructed proteoliposomes: a fluorescent analysis Voprosy Virusologii 27(4): 432-437
Gao, X.M.; Liew, F.Y.; Tite, J.P. 1989: Identification and characterization of T helper epitopes in the nucleoprotein of influenza A virus Journal of Immunology 143(9): 3007-3014
Semenova, N.P.; Prokudina, E.N. 1991: Detection of influenza virus nucleoprotein on the surface of infected cells and in free nonvirion form Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 4: 18-23
Abramova, T.V.; Vlasov, V.V.; Ivanova, E.M.; Zarytova, V.F.; Fokina, T.N.; Frolova, E.I.; Iurchenko, L.V. 1994: Suppression of influenza virus NP-protein mRNA translation in vitro with derivatives of an antisense oligonucleotide Molekuliarnaia Biologiia 28(2): 307-312
Wang, N.-F.; Ma, J.; Zhang, X.-G.; Zhang, X.-M.; Bai, T.; Wang, M.; Wen, L.-Y.; Wang, D.-Y.; Shu, Y.-L.; Zhou, L.; Zeng, Y. 2010: Expression and purification of avian influenza virus H5N1 NP protein, and screening interaction proteins of host cells in vitro Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 24(1): 27-29
Garten, W.; Vey, M.; Ohuchi, R.; Ohuchi, M.; Klenk, H.D. 1991: Modification of the cleavage activation of the influenza virus hemagglutinin by site-specific mutagenesis Behring Institute Mitteilungen 89: 12-22
Evenshteĭn, Z.M. 1976: In vitro susceptibility of the proteins of wheat-protein and bran-protein bread to attack by gastric and pancreatic proteolytic enzymes Voprosy Pitaniia 2: 58-61
L'vov, D.K. 2006: Population interactions in biological system: influenza virus A--wild and domestic animals--human; reasons and consequences of introduction high pathogenic influenza virus A/H5N1 on Russian territory Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2006(3): 96-100
Havlícková, M.; Alatortseva, H.; Plyusnin, A.; Stumpa, A. 1992: Possible use of matrix protein obtained by cloning in E. coli in the serological diagnosis of type a influenza Acta Virologica 36(3): 332
Boshchenko, I.A.; Zaĭdes, V.M.; Zhdanov, V.M. 1982: Association of influenza virus intracellular ribonucleoproteins with the nonstructural virus-specific protein NS1 Voprosy Virusologii 27(5): 47-52
Johnson, G.; Boxer, P.A.; Drummond, J.T.; Boyd, D.K.; Anderson, R.J. 1989: Identification and evaluation of O-alkyl substituted hydroxamic acids as potent in vitro inhibitors of the hepatic glycine cleavage system and investigation of their action on in vivo central nervous system glycine concentration Arzneimittel-Forschung 39(4): 432-437
Veselovskaia, T.V.; Rykova, L.A.; Selimova, L.M.; Zaĭdes, V.M. 1993: Pathological cleavage of human immunodeficiency virus gp160 protein in infected cells as a probable factor for infection becoming chronic Molekuliarnaia Biologiia 27(1): 211-221
Liuzzi, G.M.; Ventola, A.; Rizzo, T.; Riccio, P.; Quagliariello, E. 1991: Zinc as an inhibitor of myelin basic protein proteolytic breakdown in the central nervous system Acta Neurologica 13(2): 153-161
Alexandrescu, M.; Handrache, L.; Moroşanu, V.; Vancea, G.; Ionescu, L.; Colev, A. 1974: Influenza viruses and lympho-macrophagic cells. I. in vitro interaction between influenza A2-59 virus (H2N2) and peritoneal macrophagic cells from normal or immunized mice and rats Revue Roumaine de Virologie 25(1): 3-14
Hwang, L.H.; Lin, Y.J.; Lai, W.C.; Lo, M.S. 1991: Protease-like sequence in hepatitis B virus core antigen is not involved in the cleavage processes of core protein in Escherichia coli Zhonghua Minguo Wei Sheng Wu Ji Mian Yi Xue Za Zhi 24(1): 71-83
Erkell, L.J.; Schirrmacher, V. 1988: Quantitative in vitro assay for tumor cell invasion through extracellular matrix or into protein gels Cancer Research 48(23): 6933-6937
Chodynicki, S.; Worowski, K.; Popko, E. 1984: Proteolytic activity, components of the hemostatic system and protein degradation in cholesteatoma and granuloma of the middle ear in children and adults Przeglad Lekarski 41(10): 601-604