Detection of influenza virus nucleoprotein on the surface of infected cells and in free nonvirion form
Semenova, N.P.; Prokudina, E.N.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 4: 18-23
1991
ISSN/ISBN: 0208-0613 PMID: 1857375 Document Number: 384116
The inner viral nucleoprotein synthesized de novo is shown to be exposed on the surface of the chicken embryo infected with influenza virus. The kinetics of the nucleoprotein located on the surface does not correlate with the kinetics of cell destruction. In culture or allantois virus containing liquids the large number of extracellular viral nucleoprotein prone to antinucleoprotein monoclonal antibodies was found. The accumulation of this nucleoprotein occurs in the period when cell destruction is absent, it is eliminated by the adsorption of the virus on erythrocytes or centrifugation at 70,000 g for 2 hours (20%) or by centrifugation at 10,000 g for 4 hours (50%).