Limitations in the translation of influenza virus mRNAs in infected cells
Zaĭdes, V.M.; Farashian, V.R.; Boshchenko, I.A.; Zhdanov, V.M.
Biokhimiia 46(12): 2174-2178
1981
ISSN/ISBN: 0320-9725 PMID: 7317536 Document Number: 178406
The monolayer cultures of chicken embryonic cells were infected with influenza virus and 6 hrs after the infection the relative concentrations of viral mRNAs and the relative rates of virus-specific protein synthesis were determined. For this purpose the infected cells were incubated under conditions of continuous labelling of RNAs by the radioactive precursor; the mRNA fraction was obtained by hybridization of total RNA of infected cells with non-labelled RNA of viral particles and the individual mRNAs were fractionated by polyacrylamide gel electrophoresis of RNA duplexes. The proteins from infected cells were labelled by [14C] amino acids for a short time and the individual virus-specific proteins were separated electrophoretically. There was no correlation between the rates of virus-specific protein synthesis and the relative concentrations of corresponding mRNAs. This indicates the regulation of virus-specific protein synthesis at the post-translational level.