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Construction and identification of non-replication recombinant vaccinia virus co-expressing human papillomavirus type 16 L1/L2/E6/E7 proteins

Huang, W.; Tian, H-wen.; Ren, J.; Fan, J-tao.; Zhao, L.; Bian, T.; Lu, Z-hua.; Ruan, L.

Zhonghua Shi Yan He Lin Chuang Bing du Xue Za Zhi 19(3): 240-243

2005

ISSN/ISBN: 1003-9279
PMID: 16261206
Document Number: 588689
Human papillomavirus 16 (HPV16) major capsid protein L1 gene/minor capsid protein L2 gene and HPV16 early E6/E7 genes were inserted into a vaccinia virus expression vector. A strain of non-recombinant vaccinia virus containing the sequences was obtained by homologous recombination and identified. DNA hybridization confirmed that the HPV16 L1/L2/E6/E7 genes were integrated with the vaccinia virus DNA. Western blotting showed that the full-length L1/L2/E6/E7 proteins were co-expressed in CEF cells infected with the recombinant virus. It is concluded that NTVJE6E7CKL1L2 is a possible candidate prophylactic and therapeutic vaccine against HPV16-associated tumours.

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