Expression of envelope glycoprotein (E) of Japanese encephalitis virus by recombinant vaccinia virus
Haishi, S.; Imai, H.; Hirai, K.; Igarashi, A.; Kato, S.
Acta Virologica 33(6): 497-503
1989
ISSN/ISBN: 0001-723X PMID: 2576591 Document Number: 337597
Vaccinia virus recombinants inserted with cDNA clones of Japanese encephalitis (JE) virus envelope glycoprotein (E) gene were constructed. The E gene product was detected in the recombinant virus-infected BHK21 cells by immunofluorescence (IF) and Western blotting. The intensity of IF observed was higher by the recombinant of the TK promoter--P7.5 promoter--inserted cDNA construct than by the P7.5 promoter--TK promoter--inserted cDNA construct. The E gene product was hardly detected by the recombinant carrying the TK promoter only upstream to the inserted cDNA, although the glycoprotein E mRNA had been transcribed.