Expression of feline immunodeficiency virus (FIV) gag gene in vaccinia virus vector
Wojcik, J.
Acta Microbiologica Polonica 44(2): 191-196
1995
ISSN/ISBN: 0137-1320 PMID: 8906935 Document Number: 449191
The selected part of gag gene (nucleotide sequences at position 618 to 1724) coding for P24 and P15 proteins of feline immunodeficiency virus (FIV) was expressed in vaccinia virus vector under control of 7.5 kDa vaccinia virus transcriptional signal (p 7.5 vaccinia virus early/late promoter). The vaccinia virus recombinant (denoted VV-G(1)) was generated by homologous recombination following CV-1 cells transfection with a newly prepared vaccinia virus insertion vector - pWKr75. The vaccinia virus recombinant could be used in an immunoperoxidase monolayer assay as a less expensive alternative to the ELISA for FIV.
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