Two vaccinia virus recombinants expressing HBsAg with different concentration of A- and pre-S2 antigenic determinants
Chelyapov, N.V.; Rakhilina, L.E.; Antonova, T.P.; Chernos, V.I.; Grigorjev, V.G.; Chudakova, E.A.; Pashvykina, G.V.; Zakharova, L.G.; Altstein, A.D.; Kutinová, L.
Acta Virologica 35(5): 413-422
1991
ISSN/ISBN: 0001-723X PMID: 1726675 Document Number: 370189
Comparative studies of two vaccinia virus (VV) recombinants expressing the hepatitis B virus (HBV) surface antigen (HBsAg) including the pre-S2 region (M-protein) showed that the L-pre-S2/15 recombinant expressed 5-fold more HBsAg as determined by the content of a-determinant than the recombinant v137. However, both recombinants expressed comparable amounts of the pre-S2 antigenic determinant as assessed by enzyme immunoassay with monoclonal antibodies. According to our calculations, one HBsAg unit expressed by the recombinant v137 contained 7-9 times mroe pre-S2 antigen than did one HBsAg unit expressed by the L-pre-S2/15 recombinant. Binding of pre-S2 region to polymerized human serum albumin was shown not to be an efficient assay at low pre-S2 concentration. HBsAg expressed by the v137 recombinant was less extensively secreted from cells as compared to that expressed by L-pre-S2/15 recombinant. Both recombinants induced the production of antibodies to the pre-S2 antigenic determinant in rabbits. L-pre-S2/15 induced anti-HBsAg a-determinant antibody as well.