Modeling antigenic determinants of the hepatitis a virus using synthetic peptides
Kulik, L.N.; Ivanov, V.S.; Chikin, L.D.; Berkova, N.P.; Kozhich, A.T.; Gabriélian, A.E.; Ivanova, V.T.
Bioorganicheskaia Khimiia 20(7): 709-719
1994
ISSN/ISBN: 0132-3423 PMID: 7527634 Document Number: 427511
Monoclonal and polyclonal anti-hepatitis A (HAV) antibodies were used to search for peptides mimicking the antigenic determinants of HAV. Synthetic peptides VP1 115-139, VP1 117-139, VP1 126-139, VP2 69-99, VP2 80-99, VP3 45-57, VP3 137-150, were shown to bind the anti-HAV antibodies in ELISA. Peptides VP1 115-139, VP1 117-139, VP2 69-99 were utilized to produce the antipeptide antibodies. Mice were immunized with the free peptides or with their conjugates with ovalbumin. Only the free VP2 69-99 caused formation of HAV binding antibodies.