Synthesis and expression of the DNA fragment coding the antigenic determinant of the surface antigen protein of hepatitis B virus
Smirnov, V.D.; Sergienko, O.V.; Skripkin, E.A.; Dubichev, A.G.; Lunin, V.G.
Bioorganicheskaia Khimiia 9(10): 1388-1394
1983
ISSN/ISBN: 0132-3423 PMID: 6207838 Document Number: 5819
With the use of chemical-enzymatic synthesis, a polynucleotide (I) has been obtained which codes the amino acid sequence 93-109 of the hepatitis B surface antigen (HBsAg). The plasmid pHS12, constructed by insertion of (I) into pBR325 between EcoRI and BamHI sites, transformed E. coli HB101 cells. The plasmid expression in cell-free system produced a chimeric protein, its molecular weight corresponding to that a polypeptide containing the heptadecapeptide from HBsAg. An approach is discussed for creating a new type of vaccines on the basis of chimeric proteins containing the antigenic determinants of virus proteins.
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