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Genetic transformation of somatic cells. XIV. Expression of gene coding for the human hepatitis B virus surface antigen in mammalian cells

Aprelikova, O.N.; Mikhaĭlov, V.M.; Tomilin, N.V.; Bychko, V.V.; Pumpen, P.P.

Tsitologiia 29(4): 484-489

1987

ISSN/ISBN: 0041-3771
PMID: 3603707
Document Number: 303388
A set of recombinant plasmids with a gene encoding surface antigen of hepatitis B virus (HBsAg) is constructed. The plasmids were transfected by DEAE-dextran method into different lines of cultured cells and transient expression of the HBsAg gene was studied. The results indicate that: transcriptional enhancer of hepatitis B virus situated downstream from HBsAg gene is active in green monkey kidney cells (CVI), promoter of 5 LTR of bovine leukemia virus is trans-activated in the goat or calf cells infected with BLV. The results are discussed in the light of hypothesis on the role of transcriptional enhancers in determination of tissue-specificity of hepatitis B virus.

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Granovskiĭ, N.N.; Zhdanov, V.M.; Arman, I.P.; Legchilina, S.P.; Vasilenko, S.K. 1986: Gene expression of the surface antigen of the hepatitis B virus in yeast cells Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 8: 12-19
Siddiqui, A. 1983: Expression of hepatitis B surface antigen in mammalian cells and identification of a precursor form of HBsAg Developments in Biological Standardization 54: 119-123
Glebov, O.K.; Abramian, D.S.; Podgornaia, O.I.; Tomilin, N.V. 1985: Genetic transformation of somatic cells. IX. the loss of the transformant phenotype is accompanied by rearrangements in the plasmid DNA containing the thymidine kinase gene of the herpes virus Tsitologiia 27(7): 814-821
Smirnov, V.D.; Sergienko, O.V.; Skripkin, E.A.; Dubichev, A.G.; Lunin, V.G. 1983: Synthesis and expression of the DNA fragment coding the antigenic determinant of the surface antigen protein of hepatitis B virus Bioorganicheskaia Khimiia 9(10): 1388-1394
Chen, H.; Yeh, S.F.; Ong, G.T.; Wu, S.H.; Sun, C.M.; Chou, C.K. 1995: The novel desmethyldestruxin B2, from Metarhizium anisopliae, that suppresses hepatitis B virus surface antigen production in human hepatoma cells Journal of Natural Products 58(4): 527-531
Pourcel, C.; Dubois, M.F.; Rousset, S.; Chany, C.; Tiollais, P. 1981: Selection of mouse cells producing hepatitis B surface antigen after transformation with cloned viral DNA Progress in Medical Virology. Fortschritte der Medizinischen Virusforschung. Progres en Virologie Medicale 27: 93-98
Bian, Z.-q.; Liu, S.; Liu, M.-q.; Xiao, A.; Jiao, Y.; Yan, W.-y.; Zheng, Z.-x. 2012: PreC/C gene-targeting RNA interference suppresses hepatitis B virus replication and expression in human hepatoma cells Zhonghua Yi Xue Za Zhi 92(11): 768-772
Kozlovskaia, T.M.; Pumpen, P.P. 1986: Structure and expression of human hepatitis B virus surface antigen Molekuliarnaia Biologiia 20(4): 884-901
Tomilin, N.V.; Podgornaia, O.I.; Abramian, D.S.; Glebov, O.K. 1985: Genetic transformation of somatic cells. II. An analysis of the status of the plasmid nucleotide sequences in chromosomal DNA and the thymidine kinase activity in transformant clone cells Tsitologiia 27(5): 554-564
Hsie, A.W.; Brimmer, P.A.; Machanoff, R.; Hsie, M.H. 1977: Further evidence for the genetic origin of mutations in mammalian somatic cells: the effects of ploidy level and selection stringency on dose-dependent chemical mutagenesis to purine analogue resistance in Chinese hamster ovary cells Mutation Research 45(2): 271
Schettino, E.W.; Chai, S.K.; Kasaian, M.T.; Schroeder, H.W.; Casali, P. 1997: VHDJH gene sequences and antigen reactivity of monoclonal antibodies produced by human B-1 cells: evidence for somatic selection Journal of Immunology 158(5): 2477-2489
Khalizev, A.E.; Chernikov, V.G. 1976: Several technical improvements facilitating performance of genetic experiments on mammalian somatic cells Genetika 12(9): 166-169
Guo, X.; Wang, H.; Chu, H.; Wang, X.; Qu, B.; Li, Z.; Qi, Z.; Wang, Z. 2001: High level expression of human factor VIIi in mammalian cells after retroviral-mediated gene transfer Chinese Medical Journal 114(7): 690-693
Endo, K.; Borer, C.H.; Tsujimoto, Y. 1991: Modulation of LFA-1 surface antigen expression by activated H-ras oncogene in EBV-infected human B-lymphoblast cells Oncogene 6(8): 1391-1396
Sal'nikova, L.E.; Bakhitova, L.M.; Pashin, I.V. 1988: Effect of alkylation and carbamoylation on the characteristics of genetic damage to mammalian somatic cells cultured in vitro Biulleten' Eksperimental'noi Biologii i Meditsiny 105(1): 66-67
Rosenthal, K.L.; Tompkins, W.A.; Rawls, W.E. 1980: Factors affecting the expression of carcinoembryonic antigen at the surface of cultured human colon carcinoma cells Cancer Research 40(12): 4744-4750
Wolf, J.; Pawlita, M.; Bullerdiek, J.; zur Hausen, H. 1990: Suppression of the malignant phenotype in somatic cell hybrids between Burkitt's lymphoma cells and Epstein-Barr virus-immortalized lymphoblastoid cells despite deregulated c-myc expression Cancer Research 50(10): 3095-3100
Risk, J.M.; Johnson, P.M. 1985: Antigen expression by human trophoblast and tumour cells: models for gene regulation? Contributions to Gynecology and Obstetrics 14: 74-82
Khalizev, A.E.; Pyzhov, A.P.; Shapiro, N.I. 1976: Development of spontaneous mutations in somatic mamalian cells and DNA replication. II. Expression of gene mutations in cultured cells Genetika 12(11): 51-60
Kumar, A.; Mikolajczyk, S.D.; Goel, A.S.; Millar, L.S.; Saedi, M.S. 1997: Expression of pro form of prostate-specific antigen by mammalian cells and its conversion to mature, active form by human kallikrein 2 Cancer Research 57(15): 3111-3114