Expression of hepatitis B surface antigen in mammalian cells and identification of a precursor form of HBsAg
Siddiqui, A.
Developments in Biological Standardization 54: 119-123
1983
ISSN/ISBN: 0301-5149 PMID: 6418589 Document Number: 213122
The purpose of this study was to program the synthesis of hepatitis B surface antigen in cultured mammalian cells from defined DNA sequences derived from the Hepatitis B viral DNA. The product of this system used in the present study is the 22-nm hepatitis B surface antigen particles released in the culture medium with biophysical properties identical to the 22-nm HBsAg particles found in the serum of hepatitis B carriers. Additionally, preliminary results are presented concerning the synthesis of a precursor polypeptide bearing hepatitis B surface antigenic determinants.