Synthesis and expression of an artificial gene of an IgG-binding fragment of protein A from Staphylococcus aureus
Efimov, V.A.; Buriakova, A.A.; Polushin, N.N.; Pashkova, I.N.; Dmitrakova, E.V.
Bioorganicheskaia Khimiia 15(4): 499-507
1989
ISSN/ISBN: 0132-3423 PMID: 2665753 Document Number: 326725
The chemical-enzymatic syntheis of a gene for IgG-binding fragment of the staphylococcal protein A has been carried out. The design of the gene, which consists of signal peptide and modified E and B domains, and strategy of the synthesis provided possibility of various degrees of polymerization of the gene fragment coding for B domain and of the whole gene. Several protein A-like polypeptides composed of the leader sequence, E domain and 1 to 4 copies of B doamin were produced in E. coli cells under the lac promoter control.