Chemical-enzymatic synthesis, cloning and expression of a synthetic gene coding for an env protein fragment of the human T-cell leukemia virus type 1
Rosenthal, A.; Ulrich, R.; Billwitz, H.; Frank, R.; Blöcker, H.
Nucleic Acids Symposium Series 18: 233-236
1987
ISSN/ISBN: 0261-3166 PMID: 2827130 Document Number: 301958
A synthetic gene for a 88 amino acid long env protein fragment of the human T-cell leukemia virus type 1 (HTLV1) has been assembled by ligation of 35 oligodesoxyribonucleotides, which were chemically synthesized by the phosphotriester segmental support method. After cloning into the pEX vector this HTLV1 env-protein fragment was expressed in E. coli.
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