Chemical-enzymatic synthesis and cloning of the full-length genome of a DNA-containing plant virus
Chernov, B.K.; Merits, A.; Mizenina, O.A.; Morozov, S.Iu.
Bioorganicheskaia Khimiia 18(12): 1487-1495
1992
ISSN/ISBN: 0132-3423 PMID: 1301001 Document Number: 388235
The full-length genomic DNA of a small plant virus - coconut foliar decay virus (CFDV) - has been synthesized by a combination of chemical synthesis, ligation and polymerase chain reaction. Three separately cloned DNA fragments of the genomic DNA were sequenced and assembled in the pPCV002 plasmid vector.
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