Top Abstract Purchase Related
Home
>
Node
>
Subnode

An efficient chemical-enzymatic synthesis of LHRH N-terminal pentapeptide

Slomczynska, U.; Leplawy, T.; Leplawy, M.T.

Biomedica Biochimica Acta 50(10-11): S205-S208

1991

ISSN/ISBN: 0232-766X
PMID: 1820047
Document Number: 383460
Synthesis of LHRH N-terminal pentapeptide Glp1-His2-Trp3-Ser4-Tyr5-OMe (6) involving 3 + 2 enzymatic coupling has been developed. Synthetic strategy features the formation of one peptide bond (Glp-His) by chemical coupling and three peptide bonds by means of papain (Trp-Ser, Ser-Tyr) or alpha-chymotrypsin (His-Trp). High efficiency of this six-step synthesis is demonstrated by 44% overall yield. Its advantages are the use of inexpensive enzymes, simple isolation of intermediates and final pentapeptide, and easy recovery of substrates.

Document emailed within 1 workday
Secure & encrypted payments

Chernov, B.K.; Merits, A.; Mizenina, O.A.; Morozov, S.Iu. 1992: Chemical-enzymatic synthesis and cloning of the full-length genome of a DNA-containing plant virus Bioorganicheskaia Khimiia 18(12): 1487-1495
Lasta, S.; Fajloun, Z.; Mansuelle, P.; Sabatier, J.M.; Boudabous, A.; Sampieri, F. 2008: Chemical synthesis of lactococcin B and functional evaluation of the N-terminal domain using a truncated synthetic analogue Archives de l'Institut Pasteur de Tunis 85(1-4): 9-19
Rosenthal, A.; Ulrich, R.; Billwitz, H.; Frank, R.; Blöcker, H. 1987: Chemical-enzymatic synthesis, cloning and expression of a synthetic gene coding for an env protein fragment of the human T-cell leukemia virus type 1 Nucleic Acids Symposium Series 18: 233-236
Romão Júnior, J.E.; Fares, J.A.; Silva, E.C.; Fadil, M.A.; Abensur, H.; Massola, V.C.; Marcondes, M.; Sabbaga, E. 1989: Continuous ambulatory peritoneal dialysis: efficient treatment for children with terminal uremia Amb: Revista da Associacao Medica Brasileira 35(3): 103-106
Keizman, D.; Eisenberger, M.A. 2009: LHRH antagonists vs LHRH agonists: which is more beneficial in prostate cancer therapy? Oncology 23(7): 631-632
Arimura, A.; Talbot, S.; Serafini, P.C.; Schally, A.V. 1980: Sites of paradoxical action of the LHRH agonist (D-Trp6)-LHRH International Journal of Fertility 25(3): 151-156
Okuda, K.; Ushiroyama, T.; Yoshikawa, M.; Sugimoto, O. 1988: Effect of LHRH agonist (buserelin) on pulsatile secretion of LHRH and LH Nihon Sanka Fujinka Gakkai Zasshi 40(12): 1889-1893
Kuber, W.; Treu, T.; Kratzik, C.; Girsch, E.; Zeillinger, R.; Spona, J. 1990: Chemical castration using a depot LHRH-agonist as a palliative therapy concept in prostatic carcinoma--clinical, endocrinological and experimental studies Wiener Klinische Wochenschrift 102(21): 640-647
Zhu, B.; Chu, Y.; Chu, J.; Liu, Y. 1990: The direct effects of lhrh and three lhrh analogs on progesterone secretion by early pregnant human luteal cells in vitro Journal of Shanghai Medical University 17(1): 11-14
Gelormini, A.; Barbaro, M.; Cidaria, D.; Dall'Olio, M. 2005: Integration of biological and workplace monitoring as an efficient control of chemical exposure Giornale Italiano di Medicina del Lavoro Ed Ergonomia 27(3): 329-331
Lo Coco, F.; Cafolla, A.; Lopez, M.; Martelli, M.; Pasqualetti, D.; De Rossi, G. 1986: Terminal deoxynucleotidil transferase (TdT) activity in human leukemia. Quantitative evaluation with enzymatic immunoassay (EIA) Haematologica 71(4): 345-347
Jonch-Clausen, T.; Hamrin, S.; Linden, O. 2005: Workshop 6 (synthesis): water for efficient, sustainable fisheries and aquaculture Water Science and Technology: a Journal of the International Association on Water Pollution Research 51(8): 155
Wanderley, M.I.; Negro-Vilar, A. 1996: Pretreatment with phorbol ester and an LHRH agonist reduces testosterone production and protein kinase C activity in rat Leydig cells challenged with PDBu and LHRH Brazilian Journal of Medical and Biological Research 29(11): 1557-1565
Shetgiri, N.P.; Kokitkar, S.V.; Sawant, S.N. 2001: Radermachera xylocarpa: the highly efficient source of lapachol and synthesis of its derivatives Acta Poloniae Pharmaceutica 58(2): 133-135
Singh, V. 1992: Immunospecificity and affinity studies on anti-luteinizing hormone releasing hormone antibodies developed by azo-LHRH-TT and azo-LHRH-BSA conjugates Biochemistry International 27(4): 591-599
Rao, D.N.; Gurnani, S. 1972: Enzymatic and physico-chemical properties of DNP derivatives of lysozyme obtained at pH 9.i Indian Journal of Biochemistry and Biophysics 9(1): 49-51
Boccù, E.; Ebert, C.; Gardossi, L.; Gianferrara, T.; Zacchigna, M.; Linda, P. 1991: Enzymatic synthesis of ampicillin: a chemometric optimization Farmaco 46(4): 565-577
Sklyarenko, A.V.; EI'darov, M.A.; Kurochkina, V.B.; Yarotskii, S.V. 2015: Enzymatic Synthesis of Acidic β-Lactams (Review) Prikladnaia Biokhimiia i Mikrobiologiia 51(6): 546-560
Andrieux-Domont, C.; Le-van-Hung 1970: Influence of magnesium on enzymatic synthesis of coenzyme a Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 164(2): 292-296
Stozharov, A.N.; Razumovich, A.N. 1975: Physico-chemical and enzymatic properties of contractile protein of rat liver mitochondria Biokhimiia 40(6): 1145-1153