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Chemico-enzymatic synthesis, cloning and expression of a gene for an analog of human anaphylatoxin C5a

Babkina, I.N.; Seregin, S.V.; Daniliuk, N.K.; Siniakov, A.N.; Gladkova, S.E.; Pozdniakov, S.G.

Bioorganicheskaia Khimiia 21(5): 359-364

1995

ISSN/ISBN: 0132-3423
PMID: 7661861
Document Number: 451940
Chemical-enzymatic synthesis and cloning of a gene for a human anaphylatoxin C5a analog were carried out. Recombinant plasmid pRC5a providing the expression of the synthetic gene in the Escherichia coli cells was obtained. The biological activity of the expression product was demonstrated by the chemotaxis activity test and by the release of myeloperoxidases from rat peritoneal cells that was induced by bacterial cell lysates containing the recombinant protein.

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