Chemical synthesis of a human fibroblast interferon gene and its expression in Escherichia coli
Nagase, Y.; Nakamura, N.; Tohyama, J.; Watanabe, S.; Ogino, H.; Horikoshi, K.; Nii, A.; Soma, M.; Nobuhara, M.; Suzuki, Y.
Nucleic Acids Symposium Series 12: 83-86
1983
ISSN/ISBN: 0261-3166 PMID: 6364045 Document Number: 216188
Using the solid phase phosphotriester method, a gene coding for human fibroblast interferon consisting of 166 amino acid residues was chemically synthesized. The gene obtained by ligation of 61 synthesized oligodeoxyribonucleotide fragments was inserted into the downstream of tryptophan promoter, and expressed in E. coli. Lysate of this E. coli showed the antiviral activity which was specifically neutralized by anti-fibroblast interferon antibody. No particular advantage was observed in the expression efficiency by the synthetic gene over that by the native gene.