Expression in Escherichia coli of the human leukocyte interferon alpha2 gene fused with N-terminal fragment of beta-galactosidase
Petrenko, V.A.; Tat'kov, S.I.; Semenova, L.N.; Il'ichev, A.A.; Pozdniakov, P.I.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 4: 37-41
1988
ISSN/ISBN: 0208-0613 PMID: 3136326 Document Number: 309200
Genes for leucocyte interferon and alpha-donor of galactosidase were fused by deletion mutagenesis or by site-directed mutagenesis. In both cases the fused protein was expressed. The protein having an antiviral activity of leucocyte interferon was easily detected in bacteria and solutions by the reaction of beta-galactosidase alpha-complementation and retained the antigenic determinants of interferon and beta-galactosidase. The use of fused proteins for optimization of gene expression and for the analysis of interferon structure-function relationship is discussed.