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Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector

Zhou, N.; Huang, X.-p.; Liao, N.; Wei, S.-l.; Liang, F.-x.; Mai, H.-m.

Hua Xi Kou Qiang Yi Xue Za Zhi 25(5): 487-489

2007

ISSN/ISBN: 1000-1182
PMID: 18072567
Document Number: 606563
To clone human bone morphogenetic protein-2 (hBMP2) gene and construct its eukaryotic expression vector pcDNA3.1 -hBMP2. Human BMP2 gene was amplified by RT-PCR method from human osteosarcoma cells and constructed into eukaryotic expression vector pcDNA3.1-hBMP2. The gene in the vector pcDNA3.1-hBMP2 was identified by PCR amplification, enzyme digestion and DNA sequencing. The cloned DNA was confirmed to be hBMP-2 gene. In this study, hBMP2 gene is successfully cloned and its eukaryotic expression vector pcDNA3.1-hBMP2 is constructed, which provides the foundation of using BMP2 gene therapy to accelerate new bone formation in distraction osteogenesis.

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