Top Abstract Purchase Related
Home
>
Node
>
Subnode

Construction of retroviral vector with human brain-derived neurotrophic factor gene expression and in the fibroblasts expression

Yang, C.; Yan, R.; Guo, H.; Gu, X.

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 23(5): 588-591

2009

ISSN/ISBN: 1002-1892
PMID: 19514583
Document Number: 630626
To construct human brain-derived neurotrophic factor retroviral vector-pLXSN (hBDNF-pLXSN), and to evaluate the bioactivity of hBDNF. The genome mRNA was extracted from embryonic brain tissue of a 5-month-old infant, the hBDNF gene sequence was obtained with RT-PCR technology, and hBDNF-pLXSN constructed in vitro was used to infect the fibroblasts (NIH/3T3). The expression of hBDNF was identfied by the immunohistochemistry method, and the NIH/3T3 and BDNF biological activities were determined by culture of the PC12 cells and dorsal root ganglia. The hBDNF-pLXSN was constructed successfully by sequencing analyses. The infected NIH/3T3 showed positive expression of hBDNF. The infected NIH/3T3 could product hBDNF. Bioactivity of the products could support the PC12 cell survival and neurite growth in the primary cultures of dorsal root ganglia neurons of mice. hBDNF-pLXSN virus has the ability to infect NIH/3T3 and make it expressed and secreted hBDNF with the biological activity. It can be used to treat facial paralysis as a gene therapy.

Document emailed within 1 workday
Secure & encrypted payments

Yan, H.; Wang, Y.; Wang, Y.; Wang, Z.; Zheng, H. 2012: Construction and expression of bisbicistronic expression vector of novel endocrine and exocrine protein gene associated with breast cancer and IRES mediated gene EGFP Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 29(4): 737-744
Zhou, L.; Guo, K.; Li, J. 2002: Expression of human G6PD gene in K562 cells mediated by retroviral vector Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 16(4): 361-363
Chen, Z.; Li, T.; Hu, S.; Wang, G. 2009: Construction of recombinant adenovirus vector containing rat insulin-like growth factor 1 gene and its expression in islet beta-cells Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 26(5): 1067-1071
Wang, J-min.; Hou, J.; Qiu, X-fang.; Kurachi, K.; Xue, J-lun. 2004: Hybrid retroviral vector with MCK enhancers inserted in LTR for stable and specific expression of human factor IX in skeletal muscle Chinese Medical Journal 117(6): 893-898
Zhou, N.; Huang, X.-p.; Liao, N.; Wei, S.-l.; Liang, F.-x.; Mai, H.-m. 2007: Cloning of human bone morphogenetic protein-2 gene and the construction of its eukaryotic expression vector Hua Xi Kou Qiang Yi Xue Za Zhi 25(5): 487-489
Zhang, W.; He, G.; Ma, B. 2012: Construction and expression identification of human secreted apoptosis-related protein 1 gene yeast two-hybrid bait vector Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 26(12): 1492-1496
Zhong, Q.; Yan, F.-H.; Li, Y.-F.; Jiang, Y.-P. 2011: Transient expression of exogenous human platelet-derived growth factor-B in gingival fibroblasts of dog Hua Xi Kou Qiang Yi Xue Za Zhi 29(2): 214-219
Ding, C.-l.; Yao, K.; Zhang, T.-t.; Xu, J.-y.; Xu, L.; Peng, G.-y. 2003: Expression of HBcAg in eukaryotic cells by retroviral vector mediated gene transfer Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 17(1): 81-84
Guo, X.; Wang, H.; Chu, H.; Wang, X.; Qu, B.; Li, Z.; Qi, Z.; Wang, Z. 2001: High level expression of human factor VIIi in mammalian cells after retroviral-mediated gene transfer Chinese Medical Journal 114(7): 690-693
Zhou, X.; Zhang, W.; Bao, T. 1998: Expression of glial cells line-derived neurotrophic factor in the central nervous system of rat Hua Xi Yi Ke da Xue Xue Bao 29(3): 289-291; 306
Zhang, K.; Lu, D.; Xue, J.; Huang, Y.; Huang, S. 1997: Construction of mammary gland-specific expression vectors for human clotting factor IX and its secretory expression in goat milk Chinese Journal of Biotechnology 13(4): 271-276
Xiao, Y.; Wei, Q.; Liu, Y.; Wang, H. 2007: Construction of CYP1A1-Wt and Asn461, Val462 gene eukaryotic expression vector and itstransfection in HLF cells Wei Sheng Yan Jiu 36(2): 217-219
Golimbet, V.E.; Alfimova, M.V.; Korovaĭtseva, G.I.; Lezheĭko, T.V. 2014: The moderating effect of the Va166Met polymorphism of brain-derived neurotrophic factor gene on the clinical and psychological features of patients with schizophrenia Molekuliarnaia Biologiia 48(1): 81-88
Wang, J.; Wu, J.-Z.; Guo, F.-P.; Zhu, X.-L.; Wen, D.-S. 2006: Construction of eukaryotic expression vector of short hairpin RNA for transforming growth factor-beta1 Hua Xi Kou Qiang Yi Xue Za Zhi 24(2): 113-116
Cheng, X.; Lin, H.; Xue, J.; Zhang, T. 2010: Construction of human GDF-5 on adenovirus vector and its expression in MSCs Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 27(1): 120-125
Lei, K.; Che, T.-J.; Wang, J.-M.; Deng, N.; Zhang, L.; He, X.-Y. 2009: Mutation analysis of the eda-A1 gene for hypohidrotic ectodermal dysplasia and construction of recombined eukaryotic expression vector Hua Xi Kou Qiang Yi Xue Za Zhi 27(6): 610-613
Xu, Y.; Chen, L.; Zhu, X.-S.; Shi, Q.; Liu, Y.; Gu, Y.; Tang, T.-S. 2010: Construction of lentiviral vector containing SOX9 gene and its expression in bone marrow mesenchymal stem cells Zhonghua Yi Xue Za Zhi 90(47): 3376-3380
Cornetta, K.; Berebitsky, D.; Behnia, M.; Traycoff, C.; Srour, E.F.; Sledge, G.W. 1994: A retroviral vector expressing human interferon gamma upregulates MHC antigen expression in human breast cancer and leukemia cell lines Cancer Gene Therapy 1(2): 91-98
Oikarinen, A.; Vuorio, T.; Mäkelä, J.; Vuorio, E. 1989: 13-cis retinoic acid and dexamethasone modulate the gene expression of epidermal growth factor receptor and fibroblast proteoglycan 40 core protein in human skin fibroblasts Acta Dermato-Venereologica 69(6): 466-469
Wu, Z.-J.; Yang, X.-H.; Zheng, S.-S.; Yang, S.-F.; Shi, D. 2004: Construction of human eukaryotic expression plasmid vascular endothelial growth factor 165 and its expression in transfected vascular smooth muscles Hepatobiliary and Pancreatic Diseases International: Hbpd Int 3(3): 355-359