Cloning and expression of a pseudo-ovalbumin gene
Woo, S.L.; Tsai, S.Y.; Tsai, M.J.; Lai, E.C.; Mace, M.L.; O'Malley, B.W.
Biochemical and Biophysical Research Communications 89(3): 997-1005
1979
ISSN/ISBN: 0006-291X PMID: 385002 Document Number: 149476
A pseudo-ovalbumin gene, bearing significant nucleotide sequence homology to the ovalbumin gene, was cloned from genomic chick DNA. Similar to the authentic ovalbumin gene, the pseudo-gene is a unique sequence gene in the chick genome and is expressed at a low level in the immature chick oviduct. In contrast to the ovalbumin gene, expression of the pseudo-gene in the oviduct is not inducible by estrogen. The concentration of pseudo-gene RNA is only .apprx. 0.01% of that of authentic ovalbumin mRNA in estrogen-stimulated oviduct cells. Nucleotide sequence analysis of the 2 sequence related genes may reveal the molecular basis of differential response to steroid hormone induction in the same tissue.