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Molecular cloning, expression and characterization of alpha-amylase gene from a marine bacterium Pseudoalteromonas sp. MY-1

Tao, X.; Jang, M.-S.; Kim, K.-S.; Yu, Z.; Lee, Y.-C.

Indian Journal of Biochemistry and Biophysics 45(5): 305-309

2008

ISSN/ISBN: 0301-1208
PMID: 19069841
Document Number: 619131
A gene (amyA) encoding an extracellular alpha-amylase from a marine bacterium Pseudoalteromnonas sp. MY-1 was cloned and expressed in Escherichia coli. It comprised an open-reading-frame of 2,007 base pairs and encoded a protein of 669 amino acids with a predicted molecular weight of 73,770 daltons and a pI of 5.15. The entire amino acid sequence of amyA gene showed 86% similarity to the alpha-amylase preproprotein from Pseudoalteromonas haloplanktis. It consisted of a signal peptide, alpha-amylase catalytic domain and an amy C domain. The recombinant amylase was purified to homogeneity and biochemically characterized. The enzyme revealed maximum activity at pH 7.0 and 40 degrees C. The enzyme hydrolyzed soluble starch and some maltooligosaccharides to several oligosaccharides, and maltose was the common product from different substrates.

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Fukagawa, T.; Suzuki, S. 1993: Cloning of gene responsible for tributyltin chloride (TBTC1) resistance in TBTC1-resistant marine bacterium, Alteromonas sp. M-1 Biochemical and Biophysical Research Communications 194(2): 733-740
Nedashkovskaia, O.I.; Ivanova, E.P.; Bakunina, I.I.; Svetashev, V.I.; Zviagintseva, T.N.; Mikhaĭlov, V.V. 2002: Characterization of marine bacteria Pseudoalteromonas citrea degradating fucoidan Mikrobiolohichnyi Zhurnal 64(2): 3-10
Elanskaia, I.V.; Morzunova, I.B. 1989: Cloning the alpha-amylase gene of Bacillus amyloliquefaciens in cyanobacteria cells Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 9: 7-11
Cao, F.; Xia, J.; Chen, Y.-p.; Zhang, M.; Xiang, L.-e.; Zeng, J.-l.; Chen, M.; Lan, X.-z.; Liao, Z.-h. 2016: Molecular cloning and functional characterization of the gene encoding hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase gene from Artemisia annua L Yao Xue Xue Bao 51(11): 1791-1798
1985: Molecular cloning and characterization of the yeast RAD10 gene and characterization of RAD10 protein in E. coli EMBO Journal 4(13B): 3907
Hongyo, H.; Kurihara, H.; Kokeguchi, S.; Miyamoto, M.; Maeda, H.; Hayakawa, M.; Abiko, Y.; Takashiba, S.; Murayama, Y. 1997: Molecular cloning and characterization of the gene encoding 53 kD outer membrane protein of Porphyromonas gingivalis Microbios 92(370): 47-57
Jia, W.Y. 1997: Molecular cloning and expression of a dexamethasone (DEX)-induced gene in mouse thymus Hokkaido Journal of Medical Science 72(6): 597-606
Whitehead, L.A.; Stosz, S.K.; Weiner, R.M. 2001: Characterization of the agarase system of a multiple carbohydrate degrading marine bacterium Cytobios 106(Suppl): 99-117
Su, G.L.; Freeswick, P.D.; Geller, D.A.; Wang, Q.; Shapiro, R.A.; Wan, Y.H.; Billiar, T.R.; Tweardy, D.J.; Simmons, R.L.; Wang, S.C. 1994: Molecular cloning, characterization, and tissue distribution of rat lipopolysaccharide binding protein. Evidence for extrahepatic expression Journal of Immunology 153(2): 743-752
Iurchenko, I.V.; Khromov, I.S.; Budilov, A.V.; Deev, S.M.; Sobolev, A.I. 2003: Thermostability of alkaline phosphatase of Meithermus ruber bacteria: cloning of the gene, expression in Escherichia coli cells and biochemical characterization of the recombinant protein Molekuliarnaia Biologiia 37(6): 989-998
Vázquez, S.C.; Hernández, E.; Mac Cormack, W.P. 2008: Extracellular proteases from the Antarctic marine pseudoalteromonas sp. P96-47 strain Revista Argentina de Microbiologia 40(1): 63-71
Li, J.-W.; Liu, Y.; Li, K.; Song, D.; Ye, Q.-P. 2010: Cloning of a fusion gene containing hGM-CSF gene and gene encoding L protein of HBV and expression in Pichia Pastoris Hepato-Gastroenterology 57(99-100): 578-582
Ray, M.K.; Magdaleno, S.; O'Malley, B.W.; DeMayo, F.J. 1993: Cloning and characterization of the mouse Clara cell specific 10 kDa protein gene: comparison of the 5'-flanking region with the human rat and rabbit gene Biochemical and Biophysical Research Communications 197(1): 163-171
Strauch, A.R.; Min, B.; Reeser, J.C.; Berman, M.D.; Miller, H.R.; Foster, D.N. 1990: Cellular and molecular control of vascular smooth muscle alpha-actin gene expression during BC3H1 myogenic cell differentiation Progress in Clinical and Biological Research 327: 583-589
Sakanian, V.A.; Legreĭn, K.; Charlier, D.; Kochikian, A.V. 1993: N-acetylglutamate-5-phosphotransferase of the thermophilic bacterium Bacillus stearothermophilus: nucleotide sequence of the gene and enzyme characterization Genetika 29(4): 556-570
Heese, O.; Hansen, G.; Höhne, W.E.; Körner, D. 1991: A thermostable alpha-amylase from Thermoactinomyces vulgaris: purification and characterization Biomedica Biochimica Acta 50(3): 225-232
Berdal, K.G.; Bjørås, M.; Bjelland, S.; Seeberg, E. 1990: Cloning and expression in Escherichia coli of a gene for an alkylbase DNA glycosylase from Saccharomyces cerevisiae; a homologue to the bacterial alkA gene EMBO Journal 9(13): 4563-4568
Kaufman-Szymczyk, A.; Wojtasik, A.; Parniewski, P.ł; Białkowska, A.; Tkaczuk, K.; Turkiewicz, M. 2009: Identification of the csp gene and molecular modelling of the CspA-like protein from Antarctic soil-dwelling psychrotrophic bacterium Psychrobacter sp. B6 Acta Biochimica Polonica 56(1): 63-69
Becker, W.M.; Grobe, K.; Petersen, A. 1999: Cloning and characterization of Ph1 p 1 in different expression systems Arbeiten aus dem Paul-Ehrlich-Institut Zu Frankfurt A.M 93: 171-178; Discussion 179
Smirnova, N.A.; Sorokin, A.V.; Laptev, D.A.; Veĭko, V.P.; Kozlov, I.I. 1988: Two-codon mutagenesis of alpha-amylase gene of Bacillus amyloliquefaciens Molekuliarnaia Biologiia 22(5): 1265-1271