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Cloning the alpha-amylase gene of Bacillus amyloliquefaciens in cyanobacteria cells

Elanskaia, I.V.; Morzunova, I.B.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 9: 7-11

1989

ISSN/ISBN: 0208-0613
PMID: 2515431
Document Number: 339118
The recombinant plasmids of pIAH4amy series were constructed containing the alpha-amylase gene of Bacillus amyloliquefaciens A50 with its own promoter and leading sequence within an integrative vector plasmid pIAH4 (CmR) for cyanobacterium Anacystis nidulans R2. At Anacystis nidulans transformation the hybrid plasmids integrate into cyanobacterium chromosome with high efficiency and all CmR transformants produce alpha-amylase. Expression of bacillar alpha-amylase gene in cyanobacterium cells is independent of the cloned gene orientation in the vector plasmid. Secretion of alpha-amylase into the cyanobacterial periplasm has been demonstrated.

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