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Cloning and the expression of the DNA promotor fragments of Bacillus thuringiensis in Escherichia coli cells

Tsoĭ, T.V.; Sakanian, V.A.; Lebedev, A.N.; Alikhanian, S.I.

Genetika 17(12): 2100-2104

1981

ISSN/ISBN: 0016-6758
PMID: 7033045
Document Number: 181574
The promoter-containing fragments of Bacillus thuringiensis subsp. galleria 69-6 DNA have been cloned on the pGA24 vector in Escherichia coli cells. The recombinant plasmids make cells resistant to tetracycline in a wide range. The level of tetracycline-resistance does not depend on the length of a foreign insertion. New polypeptides are synthesized on the template of the recombinant plasmids in vitro. The data point out the presence in Bac. thuringiensis DNA of many genes which are able to express in E. coli cells.

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