Molecular cloning and the expression of the genes of amino acid biosynthesis of Corynebacterium glutamicum in Escherichia coli cells
Beskrovnaia, O.I.; Fonshteĭn, M.I.; Kolibaba, L.G.; Iankovskiĭ, N.K.; Debabov, V.G.
Genetika 24(7): 1153-1158
1988
ISSN/ISBN: 0016-6758 PMID: 3141247 Document Number: 323083
Cloning of genes for threonine and lysine biosynthesis from Corynebacterium glutamicum was performed in Escherichia coli cells using the plasmid vector lambda pSL5. The cloned genes are identified via complementation of thrB and lysA mutations. The gene complementing thrB of E. coli is located within a 4.1 kb EcoRI fragment of C. glutamicum chromosomal DNA. All the recombinant phasmids complementing the lysA gene of E. coli contain common 2.2 kb and 3.3 kb EcoRI C. glutamicum DNA fragments. The cloned DNA fragments hybridize with identical EcoRI fragments of C. glutamicum chromosomal DNA.