Cloning the asd and lysC genes from Cornyebacterium glutamicum
Peredel'chuk, M.I.; Bukanov, N.O.; Smirnov, I.V.; Rostova, I.V.; Fedorova, N.D.; Okorokov, A.L.; Gusiatiner, M.M.; Iankovskiĭ, N.K.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1992(5-6): 25-27
1992
ISSN/ISBN: 0208-0613 PMID: 1454080 Document Number: 403011
Plasmids carrying an asd gene from a mutant. S-(2-aminoaethyl)-L-cysteine resistant strain of Corynebacterium glutamicum were selected from a clonoteque constructed on a plasmid cloning vector pSL5 by complementation of asd mutation in Escherichia coli. Evidence has been obtained that the cloned chromosomal DNA fragment contains also a complete sequence for feed-back-resistant aspartokinase lysC gene.