Molecular cloning of interferon-gamma inducible genes from a murine pre-B cell leukemia
Gribaudo, G.; Caliendo, A.; Lembo, D.; Cavallo, R.; Landolfo, S.
Journal of Biological Regulators and Homeostatic Agents 6(4): 137-141
1992
ISSN/ISBN: 0393-974X PMID: 1296455 Document Number: 399997
In the present study, a pre-B cell leukemia L1210-C7, representing a very early stae of the B lineage, was used to characterize the molecular mechanisms exploited by IFN-gamma to modulate B cell activity. A cDNA library was prepared with poly (A)+ RNA from cells stimulated with IFN-gamma and three cDNAs clones complementary to IFN-gamma inducible mRNAs were isolated by differential screening. Of these, the 9.5 cDNA hybridized to a 2.4 kb mRNA not homologous with previously cloned IFN-gamma inducible mRNAs. Furthermore, when compared with RNAs obtained from cells of different origins (fibroblasts and T cells) the 9.5 mRNA appeared to be increased only in cells belonging to the B lineage. Taken as a whole, these results demonstrate that in leukemic pre-B cells IFN-gamma induces the expression of a gene that could be employed as specific cell activation marker. (J Biol Regul Homeost Agents, 1992; 6: 137-41).