Cloning and mapping of fourteen different DNA fragments containing Aspergillus nidulans 5S rRNA genes
Bartnik, E.; Kulik, J.; Nagieć, M.M.; Zagrodzka, M.
Acta Microbiologica Polonica 33(1): 5-10
1984
ISSN/ISBN: 0137-1320 PMID: 6205550 Document Number: 236733
Fourteen different plasmids hybridizing to Aspergillus nidulans 5S rRNA were isolated from a gene bank obtained after cloning Sau3A partial digests of A. nidulans DNA in a yeast--Escherichia coli vector, pBB29. The restriction maps of these plasmids were determined. The size of the cloned fragments was 2.7-9.5 kb, 12 of the plasmids were found to code for single 5S rRNA genes and 2 coded for 2 genes. No similarity of the sequences surrounding the 5S rRNA genes was found by restriction mapping.