Transfer of isolated nuclei into protoplasts of Aspergillus nidulans
Vágvölgyi, C.; Ferenczy, L.
Experientia 48(3): 271-272
1992
ISSN/ISBN: 0014-4754 PMID: 1547859 Document Number: 404734
Nuclei were isolated from protoplasts of a haploid auxotrophic Aspergillus nidulans strain. Transformation of protoplasts prepared from a complementary haploid auxotrophic strain with these purified nuclei resulted in both heterokaryotic and diploid colonies. The nutritionally-complementing colonies appeared at a frequency of 5 .times. 10-7 to 10-8.