Cloning and sequence analysis of genes encoding Fasciola hepatica immunodominant antigens
Mohamed, M.M.; Al-Sherbiny, M.M.; Araf, A.A.Sh.; Elmamlouk, T.H.
Journal of the Egyptian Society of Parasitology 34(3): 819-840
2004
ISSN/ISBN: 1110-0583 PMID: 15587310 Document Number: 581928
Fasciola hepatica 11 showed 75% identity with F. hepatica tegumenttal antigen (TA). The biochemical analysis of the deduced proteins was identified; in addition the predicted T- & B-cell epitopes have been also evaluated. However, histological localization of identified antigens was achieved using the IPAb (in an indirect immunoflorescent antibody assay (IFA). Results revealed that the IPAb labeled the outer glycocalyx in a characteristic, pattern, which proved that the identified antigens, were tegumental in origin and that infected Fasciola subjects induced antibodies directed mainly against tegumental components.