Cloning of the Bacillus subtilis DNA fragment containing the genes for lysine and riboflavin biosynthesis
Okunev, O.V.; Shevchenko, T.N.; Maliuta, S.S.
Genetika 20(7): 1061-1066
1984
ISSN/ISBN: 0016-6758 PMID: 6432632 Document Number: 225661
The SalI fragment of chromosomal DNA of Bacillus subtilis carrying the gene for lysine biosynthesis and the regulatory operator region (ribO) from the riboflavin gene was cloned into Escherichia coli cells. This fragment was shown to contain the gene coding for lysine synthesizing enzyme. Localization of this gene in Bac. subtili was determined. New plasmids pLRS33 and pLRB4 were constructed using pBR322; they carry a fragment homologous to pLP102 plasmid containing the operon for riboflavin biosynthesis.