Cloning in cells of Bacillus subtilis and study of the characteristics of promoter regions of DNA of Bacillus mesentericus and Phage SPO2
Bashkirov, V.I.; Vasil'ev, V.V.; Prozorov, A.A.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1: 13-20
1988
ISSN/ISBN: 0208-0613 PMID: 3128731 Document Number: 307033
11 fragments of Bacillus mesentericus and phage SPO1 DNA containing promoters were cloned in Bacillus subtilis. Nucleotide sequences of these fragments were determined and S1 mapping of transcriptional start points was performed. It was found that some fragments contained tandem or overlapping promoters. Nucleotide sequences of Bacillus mesentericus promoters were compared with known promoters from bacilli. Promoter activities were determined in different phases of cell growth. Nucleotide sequences of several promoters coincided with known sigma 43, sigma 29, sigma 28-promoters. Nucleotide sequences of other promoters differ greatly from those used by the known forms of Bacillus subtilis RNA polymerase. The existence of at least four other forms of bacilli RNA polymerase specific for these promoters is suggested.