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The role of the promoter structure in the efficiency of the expression of guanylspecific ribonucleases from Bacillus intermedius and Bacillus pumilus

Kharitonova, M.A.; Vershinina, V.I.; Morozova, O.V.; Znamenskaia, L.V.

Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 4: 15-19

2006

ISSN/ISBN: 0208-0613
PMID: 17094653
Document Number: 599653
Guanylspecific ribonucleases from B. intermedius (binase) and B.pumilus (RNase Bpu) are structural and functional homologues, and their biosynthesis is subjected to the same laws. At the same time, there are essential differences in the expression efficiency of binase and RNase Bpu genes. This was first suggested to be due to differences in nucleotide sequences of promoters of the genes. Therefore, we constructed plasmids changing each different nucleotide in binase promoter for corresponding one from RNase Bpu and vise versa. It was found that the difference in RNase Bpu and binase expression was due to the only nucleotide in RNase Bpu promoter.

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